Plug values in: (300 μL) / Solute Volume = 250.Formula: Final Volume / Solute Volume = DF.Example: Make 300 μL of a 1:250 dilution.Dilution factors are related to dilution ratios in that the DF equals the parts of solvent + 1 part. This is different than a “dilution ratio,” which typically refers to a ratio of the parts of solute to the parts of solvent, for example, a 1:9 using the previous example. The dilution factor (DF) can be used alone or as the denominator of the fraction, for example, a DF of 10 means a 1:10 dilution, or 1 part solute + 9 parts diluent, for a total of 10 parts. This way of expressing a dilution as a ratio of the parts of solute to the total number of parts is common in biology. (Final Volume / Solute Volume) = Dilution Factor (can also be used with mass) To make a dilute solution without calculating concentrations, you can rely on a derivation of the above formula: Answer: Place 1.25 mL of the 1 M solution into V 1-V 2 = 5 mL – 1.25 mL = 3.75 mL of diluent.Example: Make 5 mL of a 0.25 M solution from a 1 M solution.C 2 = Final concentration of new solution.V 1 = Volume of stock solution needed to make the new solution.To make a fixed amount of a dilute solution from a stock solution, you can use the formula: C 1V 1 = C 2V 2 where:
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